Journal: EMBO Reports

Article Title: Mutant p53 regulates a distinct gene set by a mode of genome occupancy that is shared with wild type

doi: 10.1038/s44319-025-00375-y

Figure Lengend Snippet: Reagents and tools table

Article Snippet: FITC-conjugated anti-mouse lineage cocktail (B220, CD3, CD11b, Gr1, Ter119) , eBioscience , #50-112-3250.

Techniques: Sequencing, Protease Inhibitor, Purification, Software, Irradiation, Real-time Polymerase Chain Reaction, Sonication

Journal: bioRxiv

Article Title: Machine learning engineered PoLixNano nanoparticles overcome delivery barriers for nebulized mRNA therapeutics

doi: 10.1101/2024.11.03.621713

Figure Lengend Snippet: a, Endpoint titers of RBD-specific IgG antibody within serum samples of vaccinated mice collected on day 42 and day 380 measured by ELISA (n=5 biologically independent animals). b , Representative flow cytometry plots showing proportion of germinal center B cells (GCB, CD19+B220+CD95+Gr1+) and T follicular helper cells (Tfh, CD4+CXCR5+PD-1+) in mediastinal lymph nodes (MLNs) of mice vaccinated by indicated formulations. c , Endpoint titers of RBD-specific sIgA antibody in BALF and NLF samples of vaccinated mice collected on day 42 (n=5 biologically independent animals). d , Pseudo-virus neutralizing antibody titer in the BALF and serum samples from PoLixNano vaccinated mice on day 42 post nebulization (n=5 biologically independent anmimals). e , ELISpot assay for the quantification of RBD-specific IgG antibody secreting cells (ASCs) in spleen and bone marrow (BM) and RBD-specific IgA ASCs in spleen collected from vaccinated mice on day 35 (n=3 biologically independent samples). Inserts: imagines of each well. f , A heatmap showing the expression of genes related to the IFN signaling pathway in lung samples collected at 24 h and 28 days post-prime. Lung samples form mice treated with PBS served as the control (Con). A one-way ANOVA with Tukey’s post-hoc test was used for statistical analysis in a , c and e .

Article Snippet: Cell samples were incubated with cell staining buffer containing anti-mouse-CD3e-eFluor TM 506 (69-0032-82, Invitrogen, 1:200), anti-mouse-CD185(CXCR5)-APC (17-7185-82, Invitrogen, 1:200), anti-mouse-CD279 (PD-1)-PE (12-9985-82, Invitrogen, 1:100), anti-mouse-CD4-PercpCy5.5 (45-0042-82, Invitrogen, 1:100) and anti-mouse-CD44-FITC (11-0441-82, Invitrogen, 1:100) at 4 ℃ for 30 min. For GCB cell analysis, cell suspensions were incubated with a mixture of anti-mosue-CD95-PE (12-0951-81, Invitrogen, 1:100), anti-mouse-Gr1-FITC (11-5931-82, Invitrogen, 1:100), anti-mouse-B220-V450(48-0452-82, Invitrogen, 1:100), and anti-mouse-CD19-APC (17-0913-82, Invitrogen, 1:100) at 4 °C for 40 min. To quantify the alveolar macrophages (AMs) and interstitial macrophages (IMs), single-cell suspensions were prepared from BALF of immunized mice collected on day 42 post-immunization, stained with the biotin-anti-mouse-Ly-6C (128003, Bio-Legend, 1:50) for 40 min at 4 °C, and subsequently incubated with the following antibodies in cell staining buffer containing anti-mouse-CD11B-PE (12-0112-8, Invitrogen, 1:100), anti-mosue-Ly6G-APC-eFluor™780 (47-9668-82, Invitrogen, 1:100), anti-mosue-CD11C-FITC (11-0114-82, Invitrogen, 1:100), anti-mouse-MHC II-eFluor™450 (48-5321-82, Invitrogen, 1:100), anti-mouse-CD24-SuperBright 600 (63-0242-82, Invitrogen, 1:100), anti-mouse-CD64-Percp-eFluor TM 710 (46-0641-82, Invitrogen, 1:100), anti-CD170 (SiglecF)-PE-Cy7 (25-1702-82, Invitrogen, 1:100), and APC streptavidin (405307, Bio-Legend, 1:100) for 30 min at 4 °C.

Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Virus, Enzyme-linked Immunospot, Expressing, Control